Nucleic Acid Vaccines
A nucleic acid vaccine is a vaccine that uses gene delivery methods, such as lipid nanoparticles or viral vectors, to deliver some quantity of either DNA or RNA into a cell. The cell’s own machinery, in the form of RNA polymerases and ribosomes, uses these nucleic acids as instructions to synthesize proteins. In the case of a nucleic acid vaccine, the protein in question is usually one of the structural proteins of a virus, with the aim of generating an antibody response against that specific protein, but this isn’t the only type of product that nucleic acid transfection can produce. Gene transfection into cells can, in fact, make those cells produce any kind of protein, with the right instructions, including monoclonal antibodies, designer receptors, anything imaginable.
In the case of the COVID-19 vaccines, the media and the medical establishment tried getting around this by arguing that since the vaccines did not change the recipient’s DNA, that meant that they weren’t gene therapy. The introduction of foreign nucleic acids into the body to generate foreign proteins is, by definition, gene therapy, regardless of whether or not the subject’s own genes are changed by it. DNA and RNA are genetic material, and if the immune system catches a cell producing non-human proteins, some seriously bad things will happen to that cell.
Unlike a virus, which only binds to specific host factors expressed by specific cell lines and is endocytosed in those specific cells, cationic lipids, like the LNPs used in mRNA vaccines, are capable of transfecting basically any type of cell with instructions to make proteins. LNPs were investigated for many years as a means of delivering Alzheimer’s drugs to the brain, because they readily bypass the blood-brain barrier.
When the thing being delivered is a toxin, like SARS-CoV-2 Spike, however, there are serious consequences.
MDPI - A Case Report: Multifocal Necrotizing Encephalitis and Myocarditis after BNT162b2 mRNA Vaccination against COVID-19
The current report presents the case of a 76-year-old man with Parkinson’s disease (PD) who died three weeks after receiving his third COVID-19 vaccination. The patient was first vaccinated in May 2021 with the ChAdOx1 nCov-19 vector vaccine, followed by two doses of the BNT162b2 mRNA vaccine in July and December 2021. The family of the deceased requested an autopsy due to ambiguous clinical signs before death. PD was confirmed by post-mortem examinations. Furthermore, signs of aspiration pneumonia and systemic arteriosclerosis were evident. However, histopathological analyses of the brain uncovered previously unsuspected findings, including acute vasculitis (predominantly lymphocytic) as well as multifocal necrotizing encephalitis of unknown etiology with pronounced inflammation including glial and lymphocytic reaction. In the heart, signs of chronic cardiomyopathy as well as mild acute lympho-histiocytic myocarditis and vasculitis were present. Although there was no history of COVID-19 for this patient, immunohistochemistry for SARS-CoV-2 antigens (spike and nucleocapsid proteins) was performed. Surprisingly, only spike protein but no nucleocapsid protein could be detected within the foci of inflammation in both the brain and the heart, particularly in the endothelial cells of small blood vessels. Since no nucleocapsid protein could be detected, the presence of spike protein must be ascribed to vaccination rather than to viral infection. The findings corroborate previous reports of encephalitis and myocarditis caused by gene-based COVID-19 vaccines.
There has been a major push for the adoption of nucleic acid vaccine tech in prior years, largely hidden from the public eye. In order to begin tracing it out, one must simply perform date range searches for the years prior to 2020, for nucleic acid vaccines. The cheerleaders of this technology immediately reveal themselves.
Nature - mRNA vaccines — a new era in vaccinology
mRNA vaccines represent a promising alternative to conventional vaccine approaches because of their high potency, capacity for rapid development and potential for low-cost manufacture and safe administration. However, their application has until recently been restricted by the instability and inefficient in vivo delivery of mRNA. Recent technological advances have now largely overcome these issues, and multiple mRNA vaccine platforms against infectious diseases and several types of cancer have demonstrated encouraging results in both animal models and humans. This Review provides a detailed overview of mRNA vaccines and considers future directions and challenges in advancing this promising vaccine platform to widespread therapeutic use.
Frontiers - Advances in mRNA Vaccines for Infectious Diseases
During the last two decades, there has been broad interest in RNA-based technologies for the development of prophylactic and therapeutic vaccines. Preclinical and clinical trials have shown that mRNA vaccines provide a safe and long-lasting immune response in animal models and humans. In this review, we summarize current research progress on mRNA vaccines, which have the potential to be quick-manufactured and to become powerful tools against infectious disease and we highlight the bright future of their design and applications.
International Journal of Nanomedicine - Development of nucleic acid vaccines: use of self-amplifying RNA in lipid nanoparticles
Self-amplifying RNA or RNA replicon is a form of nucleic acid-based vaccine derived from either positive-strand or negative-strand RNA viruses. The gene sequences encoding structural proteins in these RNA viruses are replaced by mRNA encoding antigens of interest as well as by RNA polymerase for replication and transcription. This kind of vaccine has been successfully assayed with many different antigens as vaccines candidates, and has been shown to be potent in several animal species, including mice, nonhuman primates, and humans. A key challenge to realizing the broad potential of self-amplifying vaccines is the need for safe and effective delivery methods. Ideally, an RNA nanocarrier should provide protection from blood nucleases and extended blood circulation, which ultimately would increase the possibility of reaching the target tissue. The delivery system must then be internalized by the target cell and, upon receptor-mediated endocytosis, must be able to escape from the endosomal compartment into the cell cytoplasm, where the RNA machinery is located, while avoiding degradation by lysosomal enzymes. Further, delivery systems for systemic administration ought to be well tolerated upon administration. They should be safe, enabling the multiadministration treatment modalities required for improved clinical outcomes and, from a developmental point of view, production of large batches with reproducible specifications is also desirable. In this review, the concept of self-amplifying RNA vaccines and the most promising lipid-based delivery systems are discussed.
Nature Gene Therapy - The promise of nucleic acid vaccines
Establishing the effective use of ‘naked’ nucleic acids as vaccines would undoubtedly be one of the most important advances in the history of vaccinology. While nucleic acids show much promise for use as vaccine vectors in experimental animals, not a single naked nucleic acid vector has been approved for use in humans. Indeed, data from human clinical trials is scant: nucleic acid vaccines have not been clearly demonstrated to have any convincing efficacy in the prevention or treatment of infectious disease or cancer. Here we illustrate possible mechanisms underlying effective nucleic acid vaccination. We focus on progress that has been made in the improvement of their function. Additionally, we identify promising new strategies and try to forecast future developments that could lead to the real success of nucleic acid vaccines in the prevention and treatment of human disease.
Cell Press Molecular Therapy - Self-Amplifying RNA Vaccines Give Equivalent Protection against Influenza to mRNA Vaccines but at Much Lower Doses
New vaccine platforms are needed to address the time gap between pathogen emergence and vaccine licensure. RNA-based vaccines are an attractive candidate for this role: they are safe, are produced cell free, and can be rapidly generated in response to pathogen emergence. Two RNA vaccine platforms are available: synthetic mRNA molecules encoding only the antigen of interest and self-amplifying RNA (sa-RNA). sa-RNA is virally derived and encodes both the antigen of interest and proteins enabling RNA vaccine replication. Both platforms have been shown to induce an immune response, but it is not clear which approach is optimal. In the current studies, we compared synthetic mRNA and sa-RNA expressing influenza virus hemagglutinin. Both platforms were protective, but equivalent levels of protection were achieved using 1.25 μg sa-RNA compared to 80 μg mRNA (64-fold less material). Having determined that sa-RNA was more effective than mRNA, we tested hemagglutinin from three strains of influenza H1N1, H3N2 (X31), and B (Massachusetts) as sa-RNA vaccines, and all protected against challenge infection. When sa-RNA was combined in a trivalent formulation, it protected against sequential H1N1 and H3N2 challenges. From this we conclude that sa-RNA is a promising platform for vaccines against viral diseases.
Again and again, the same properties are touted; easy, rapid, cost-effective development and manufacture. Plug in a gene sequence for the targeted antigen and away you go.
Naturally, the military would be interested in this technology for quickly vaccinating large populations of people against bioweapons ahead of pandemic spread, because it offers the potential for rapid development and deployment of countermeasures in a wartime scenario with equally rapid-developed bioweapons being flung all over the place.
ADEPT is a DARPA program that began in 2012. The acronym stands for Autonomous Diagnostics to Enable Prevention and Therapeutics. PROTECT is a sub-program of ADEPT, and it stands for Prophylactic Options to Environmental and Contagious Threats.
Some quick searches reveal presentation slides about the project:
Autonomous Diagnostics to Enable Prevention and Treatment (ADEPT)
Rapid, distributed diagnostics, vaccines, and therapeutics brought to the warfighter.
Controlling Cellular Machinery - Vaccines Antigens, immunomodulating elements, and pharmacokinetics encoded in RNA vaccines
PROTECT provides prophylactic protection against disease by treating people with nucleic acid constructs that encode protective monoclonal antibodies.
DARPA pioneered the use of the body as a bioreactor to produce prophylactic antibodies to protect against biothreats
Apparently, the goal of ADEPT: PROTECT was to come up with nucleic acid delivery systems that encoded monoclonal antibodies (or mAbs) against specific pathogens that may be used in biowarfare, such as Influenza, Smallpox, SARS, Chikungunya, Rabies, Anthrax bacteria, and even Ricin, nerve agents, and prions.
Antibodies are the means by which the adaptive immune system tags things for destruction and disposal. They lock over the surface proteins of pathogens and guide inactivated viruses and bacteria into leukocytes, encourage complement activation, and so on. Monoclonal antibodies are essentially copies of one specific kind of antibody, for therapeutic use. This differs slightly from how mRNA vaccines have ended up being used; generating the target antigen protein instead, and letting the body manufacture antibodies against it.
In DARPA’s own words, they partnered with Moderna to produce mRNA-1944, a nucleic acid-encoded mAb against chikungunya:
In fact, DARPA openly brag on Twitter that Moderna’s mRNA vaccine tech - and, by extension, mRNA-1273, was a product of ADEPT:
Another company involved in ADEPT is Ichor Medical Systems, a little-known company in San Diego specializing in electroporation gene delivery tech that partners with Pfizer, Janssen, and USAMRIID.
Ichor Awarded DARPA ADEPT: PROTECT Contract
Ichor Medical Systems of San Diego has been awarded a contract through the Defense Advanced Research Projects Agency (DARPA) and supported by the U.S. Army Research Office for up to $20.2 million of funding over five years, including a base period award of $8.6M.
The award is part of a DARPA program called Autonomous Diagnostics to Enable Prevention and Therapeutics: Prophylactic Options to Environmental and Contagious Threats (ADEPT: PROTECT) aimed at developing new platform technologies that could be safely and rapidly deployed to the U.S. population and military personnel to provide immediate protection in the event of an infectious outbreak or biological weapons attack.
The program will fund the development and clinical assessment of Ichor’s TriGrid electroporation system as a DNA-based antibody delivery platform to produce protective antibodies for passive immunoprophylaxis.
What’s really going on, here? Why haven’t the media extensively covered the military think tank side of all of this, as well as DARPA’s enduring partnership with Moderna?
Moderna failed to disclose federal funding for vaccine patent applications, advocates say
An advocacy group has asked the Department of Defense to investigate what it called “an apparent failure” by Moderna (MRNA) to disclose millions of dollars in awards received from the Defense Advanced Research Projects Agency in patent applications the company filed for vaccines.
In a letter to the agency, Knowledge Ecology International explained that a review of dozens of patent applications found the company received approximately $20 million from the federal government in grants several years ago and the funds “likely” led to the creation of its vaccine technology. This was used to develop vaccines to combat different viruses, such as Zika and, later, the virus that causes Covid-19.
In arguing for an investigation, the advocacy group maintained Moderna is obligated under federal law to disclose the grants that led to nearly a dozen specific patent applications and explained the financial support means the U.S. government would have certain rights over the patents. In other words, U.S. taxpayers would have an ownership stake in vaccines developed by the company.
There is an extensive paper trail, here, one that shows that Moderna is just another front in the Biodefense Mafia. The media, with few exceptions, are largely silent on this matter. Now, it may seem, at first, that all of this would suggest that the ADEPT program was protective in intent. There’s just one problem with that idea; the thing people are presently being inoculated with is a deadly toxin that’s killing people.
If we are at war - and at this point, only an idiot would fail to see that we are - then who fired the first shot? Why are world leaders so tight-lipped about all of this? Well, it’s simple, really.
The reason why you have been kept in the dark is because you are the target of a globe-spanning military operation, with population reduction, mass surveillance, tyrannical control of people’s movements, and the destruction of human autonomy through implanted technology as its end goal.
In all of the affluent nations operating under the globalist managerial rules-based order of private-public partnerships, NGOs, and supranational organizations, the only real threats to the ruling class are resurgent nationalism, populism, and traditionalism, because these things invariably lead to protectionist economic policies that divert resources away from the already magnificently wealthy ruling class and towards the middle.
Populism is only a problem for the rich and powerful if there are people to embody it. No people, no problem. Hence the reliance on bioweapons and poisonous vaccines. The Neo-Malthusian ruling class want to kill off insolent, rebellious, resource-overconsuming plebeians, keep the valuable infrastructure intact, and profit off of it, after they corral just enough survivors to keep their global consumerist orgy going.
They aren’t even discreet about it. They openly revel in their extraordinarily grandiose ideas.
These people want to control you, and if they can’t control you, then they want to replace you with someone they can. The word for this condition is megalomania.
meg·a·lo·ma·nia ˌme-gə-lō-ˈmā-nē-ə -nyə
: a mania (see MANIA sense 2a) for great or grandiose performancean outburst of wildly extravagant commercial megalomania The Times Literary Supplement (London)
: a delusional mental illness that is marked by feelings of personal omnipotence and grandeur
megalomaniac ˌme-gə-lō-ˈmā-nē-ˌak adjective or noun
megalomaniacal ˌme-gə-lō-mə-ˈnī-ə-kəl adjective or less commonly megalomanic ˌme-gə-lō-ˈma-nik
megalomaniacally ˌme-gə-lō-mə-ˈnī-ə-k(ə-)lē adverb
We must throw all of our energy into exposing these people and dismantling their tyrannical institutions. As they have clearly violated the social contract and are scrambling desperately to conceal that fact, there is no point whatsoever in obeying them. Their authority is now illegitimate.
We have every right to defend ourselves and our kin from murderous despots who plot against us in the dark.
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I'm throwing my energy into surviving and helping others survive, and part of that is identifying and understanding the threats to survival.
Environments kill humans, true, but most often it's humans killing humans.
All this new technology is a Pandora's box, opened by our sociopathic owners and their expert consultants.
We will need food, water, fuel and a somewhat stable community to survive this decade.
I don't anticipate the internet and grid always being operational.
Spartacus I must commend you; this is your best work yet. It truly tries to pull all the pieces together in a narrative. I needed this this morning! The breadth of your topics and the way you pulled the together are spot on.
Just want to update everyone while I have the chance on the work of coronaized.com:
1. Please watch the new coronaized.com videos:
2. Also, coronaized.com has new papers for download and some recent updates to the Why Covid section. Check it out here: www.coronaized.com
3. Also, everyone should be alerted and start watching very closely the work of Jia Lui, who has essentially taken over the work of one Charles M. Lieber. This author has identified some of the key technology that worked the best from the first round of nanotech injections, which Mr. Lui, has, thorough Harvard, gone and patented, all the fruit of Lieber and his work. I believe this to be the antenna and organoid system utilized in the Jannsen shots. Please see and compare the following:
and compare the images and patters to the optical microscopy pictures of Dr. Pablo Campra (pp. 35-40) here:
As usual most of this is being run out of Cambridge, MA, really a filthy hive of transhumanist/eugenist/ technocrats since the end of World War II. See Jia Liu's new group page here:
Please be sure to look at the second video in the list above, where I break down the baton being handed over from Lieber to Liu and the patenting of COVID 19 tech. I will also be doing a video in the near future on Ralph Baric's recent patent filings which tie the development of SARS2 directly to its use as a molecular machine of synthetic biology, not just a VLP. Let me be clear, Lieber and Harvard University are heavily behind all the technology being injected into us to either kill or monitor us, one or the other. The ones that are killed are considered collateral damage to get to the Übermensch singularity stage in their transhumanist technocratic nightmare they want force on all of us, mostly out of a need to fill the vacuum of their own self-hatred. They genuinely believe they can hijack what they conceive to be evolution and reach godhood and a society that runs like a machine. These maniacs must be stopped. I call once again for a seizure of all lots of all the COVID-19 "vaccines" immediately and that serious scientific study, using micron and nano scale microscopy take place immediately. All politicians should be asked at every event what their position on transhumanism is and whether or not they support a full investigation into the composition of these "vaccines" using the most cutting-edge microscopic techniques with full disclosure of all ingredients and other materials in these shots. If they do not, they are nothing more than transhumanist-technocrat-controlled scum, whatever their rationale may be. Everyone is worried about symptoms of what is clearly a deadly shot but making no effort to fully examine any of these so-called vaccines in any empirical scientific manner and do the assiduous scientific study that was never undertaken by our captured governments. Essentially, most of the movement against this campaign to take over humanity attack symptoms of the problem and not the core. This needs to stop! We need samples of all these vaccines, taken from multititle lots, especially ones left from the initial roll out. and try to pick up on the work of Dr. Campra and at least verify some of his findings. Especially when it is clear there are patents with technology, with optical microscopy pictures appended as exhibits, which look almost exactly like what he uncovered using the same level of microscopy.
Please I urge anyone in this community with access to the highest tech. to please go get samples and do your examinations before it is too late. Release all your findings publicly so that no corrupt politician or bureaucrat can bury it. The only way to full expose this is with scientific evidence that has been verified by actual verifiable scientific study. The admissions of the WEF puppets like Harari cannot go unchecked. They are bragging about invading all of us "under the skin" for a reason. We need to get the evidence to put these people away for good. Nuremberg 1 failed to do this allowing most of the monsters behind Hitler (IG Farben Cartel) to go free, in addition to most radical and advanced Nazis in operations like Paperclip, and that is why we are here. Anyone who wants to assist in my efforts, please contact me at: www.coronaized.com. I will be doing what I can and filing FOIAs and the rest to trace the NIH funding and reporting, but we need real scientists with access to high tech. to crack this before we all are doomed to like in a completely controlled system where, if you are unfortunate enough to survive, your own mind will be controlled for your entire life by a form of AI. We cannot let this happen to humanity.
Thank you again Spartacus for your efforts and guidance. May God be with us all.
- Author and Creator of www.coronaized.com